Extended Data Fig. 3 |. Characterization of the interaction between NTS-NTSR1 and G_i in cNDs by two-dimensional ¹H, ¹⁵N-TROSY HSQC NMR spectroscopy.

a-b, NMR spectrum of ¹⁵N-labeled NTS-NTSR1 in cNDs in the (a) absence and (b) presence of G_i. c, Overlay of (a) (red) onto (b) (black) showing structural and dynamical changes of NTS-NTSR1 upon binding to G_i in cNDs. d, A region showing conformational stabilization of NTSR1. More peaks are observed in the presence of G_i, suggesting that NTSR1 is highly dynamic in the absence of G_i and resonances are averaged out among a wide range of conformers resulting in low signal-to-noise ratio and even disappeared peaks. Upon interaction with G_i, NTSR1 is stabilized into fewer conformers and becomes less dynamic, which leads to better signal-to-noise ratio and more resonances being observed. e, A region showing dynamically slow-exchange shift of NTSR1 upon interaction with G_i. f, A region showing chemical shift perturbation of NTSR1, suggesting conformational change of NTSR1 upon binding to G_i in cNDs.