TABLE 2.
Comparative transcriptomic analysis of carotenoid biosynthesis for the heterotrophically -grown S. acuminatus cells subjected to the high light and N-limited conditions.
| Gene_ID | KO_ID | Enzyme | Gene_name | 6 h |
12 h |
24 h |
|||
| Log2FC | FDR | Log2FC | FDR | Log2FC | FDR | ||||
| c4751_c0_g1 | K00514 | Zeta-carotene desaturase | ZDS | –1.663 | 1.72E-59 | –1.134 | 2.13E-32 | –1.669 | 1.18E-42 |
| c5887_c0_g1 | K02291 | Phytoene synthase | PSY | –1.985 | 3.22E-30 | –3.587 | 4.94E-104 | –4.356 | 2.92E-75 |
| c6666_c0_g1 | K09839 | Violaxanthin de-epoxidase | VDE | 0.239 | 5.88E-02 | 0.089 | 6.17E-01 | –1.587 | 1.70E-06 |
| c6677_c0_g1 | K09838 | Zeaxanthin epoxidase | ZEP | 0.247 | 2.96E-04 | –0.606 | 6.36E-13 | 0.021 | 8.49E-01 |
| c7062_c5_g1 | K06444 | Lycopene epsilon-cyclase | LCYE | –1.518 | 4.14E-15 | –2.820 | 3.13E-40 | –3.642 | 3.45E-14 |
| c7246_c6_g1 | K06443 | Lycopene beta-cyclase | LCYB | –1.736 | 4.89E-09 | –0.407 | 4.41E-02 | –1.213 | 1.74E-04 |
| c7438_c7_g1 | K09836 | Beta-carotene/zeaxanthin 4-ketolase | BKT | –1.105 | 2.42E-22 | –1.203 | 4.71E-16 | –0.049 | 8.33E-01 |
| c7533_c5_g3 | K09843 | (+)-abscisic acid 8′-hydroxylase | CYP707A | –0.075 | 7.97E-01 | 1.086 | 1.96E-04 | 2.393 | 3.11E-10 |
| c7684_c6_g6 | K09843 | (+)-abscisic acid 8′-hydroxylase | CYP707A | –0.989 | 5.48E-50 | –0.738 | 2.81E-17 | –0.239 | 6.21E-02 |
| c7728_c5_g5 | K15747 | Beta-ring hydroxylase | CYP97A3 | –1.097 | 1.36E-04 | –1.876 | 4.64E-15 | –3.293 | 2.94E-19 |
| c7760_c4_g2 | K09837 | Carotenoid epsilon hydroxylase | CYP97C1 | –2.136 | 3.69E-24 | –2.080 | 4.15E-15 | NA | NA |
| c7813_c9_g1 | K15745 | Phytoene desaturase (3,4-didehydrolycopene-forming) | PDS | –1.884 | 2.28E-12 | –1.884 | 5.20E-07 | NA | NA |
| c8132_c5_g1 | K15746 | Beta-carotene 3-hydroxylase | CHYB | 0.287 | 3.37E-01 | –2.342 | 1.29E-24 | –3.858 | 2.42E-22 |
| c8212_c6_g1 | K15744 | Zeta-carotene isomerase | ZISO | –2.038 | 1.65E-28 | –0.906 | 4.31E-05 | NA | NA |
| c8551_c2_g1 | K02293 | Phytoene desaturase | PDS | –1.271 | 7.44E-84 | –0.757 | 1.42E-24 | –2.526 | 2.40E-181 |
Log2FC is Log2 Fold Change. Positive value means upregulated and negative value means downregulated, NA means without detected gene.