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. 2021 May 20;14:582497. doi: 10.3389/fnmol.2021.582497

Figure 2.

Figure 2

Effects of lipopolysaccharide (LPS) and spinal cord injury (SCI) lysates in microglial cell count and metabolic activity. (A) Representative images of microglial cells activated by IFN-γ at 10 ng/ml, IL-4 at 10 ng/ml and SCI lysate at 50 μg/ml, 24 h after treatment. Scale bar: 50 μm. (B) Immunochemical images depicting labeling of Iba1 (green) and DAPI (blue) with increasing concentration of SCI lysate in the medium at 10, 25 and 50 μg/ml. Scale bar: 100 μm. (C) Higher magnification images of control microglia, and microglia activated by LPS or SCI lysate (50 μg/ml), labeled with anti-Iba1 (green) and DAPI (blue). Scale bar: 100 μm. (D) Graph showing microglia cell counts and MTT activity at 24 h upon being exposed to LPS (10 ng/ml), different concentrations of SCI lysate (10, 25, 50 and 100 μg/ml), and a combination of SCI lysate (50 μg/ml) with ibuprofen at 0.5 and 1 μM. Values are expressed as mean and SEM (*P < 0.05). n = 3 biological replicates per condition.