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. 2021 May 21;12:672415. doi: 10.3389/fimmu.2021.672415

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Copyright © 2021 Zhang, Lohani, Cheng, Wang, Guo, Kim, Gorantla and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental timeline and Flow Cytometric evaluation of human immune cells reconstitutions in the peripheral blood of hu-BLT-hIL34 and hu-BLT mice. (A) Experimental design and timeline for the humanization of hIL34 transgenic mice and NOG mice by engrafting with human CD34+ hematopoietic stem and progenitor cells (HCSPs) and human fetal liver and thymic tissues, HIV-1 Ada infection of some mice in each group, and the evaluation of human myeloid and microglial cell reconstitution and HIV-1 infection in the CNS of euthanized mice using immunohistochemical staining (IHCS) and RNAscope in situ hybridization (ISH). (B) The representative flow cytometric dot plots of the peripheral blood mononuclear cells of a hu-BLT-hIL34 mouse (#1703) at the 4 months after transplantation, which were gated with hCD45+, hCD3+, hCD9+, and hCD14+ cells. (C) The percentage of hCD14+ myeloid cells in the peripheral blood of hu-BLT-hIL34 mice is significantly higher than that hu-BLT mice at the 4 months post transplantation. Each symbol represents an individual mouse, hu-BLT-hIL34 mice n=6, hu-BLT mice n=10; ****p < 0.0001.