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. 2021 May 21;12:672415. doi: 10.3389/fimmu.2021.672415

Figure 4.

Figure 4

The microglia-specific marker hTMEM119+ microglia in the brain tissues of hu-BLT-hIL34 and hu-BLT mice and humans. A representative whole brain tissue section (C) from the third coronal brain slice of a hu-BLT-hIL34 mouse (#1703) that was stained immunohistochemically for hTMEM119+ cells (brown) and counterstained with hematoxylin. LV stands for lateral ventricle. The blue and red boxed regions in the Figure (C) were highlighted at a higher magnification (A, D). In turn, the blue and red boxed regions of the Figures (A, D) were highlighted (B, E). The hTMEME119+ cells (brown) are morphologically ramified and distributed in brain parenchyma. The histogram of quantitative images analysis of hTMEM119 + cells in the cerebral cortex of the hu-BTL-hIL34 mice (F) (304.08 ± 131.93 hTEME119+ cells/mm2, n=6), where there were an absent of these cells in the hu-BLT mice. The middle panel (G–I) shows hTMEM119 + microglia in the cerebral cortex of a HIV-1 non-infected individual with no registered medical complications (G–I). The frequency, distribution, and morphology of hTMEM119+ human microglia in hu-BLT-hIL34 mice are similar to this human individual. The lower panel (J–L) shows no detectable hTMEM119+ cells in a representative whole brain section of a hu-BLT mouse (#1721).