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. 2021 May 21;12:672415. doi: 10.3389/fimmu.2021.672415

Figure 5.

Figure 5

The HIV-1 infection of human microglia of hu-BLT-hIL34 mice. The Upper panel shows HIV-1 RNA+ cells (A, B, red) in a representative whole brain tissue section from a hu-BLT-hIL34 mouse (#1708) that was detected using RNAscope in situ hybridization with HIV-1 clade B anti-sense probe and counterstained with hematoxylin. The highlighted boxed region from the Figure (A) was shown at a higher magnification of HIV-1 RNA+ cells (B, red). HIV-1 p24+ cells (C, brown) in the cerebral cortex of the same mouse were detected using IHCS. The colocalization of HIV-1 vRNA and human myeloid cell marker hIba-1 (D, arrows) indicating the reconstituted human myeloid cells could support HIV-1 replication. The lower panel (E, F) shows there were no detectable HIV-1 RNA+ cells in a representative whole brain tissue section from a hu-BLT mouse (#1724) using RNAscope in situ hybridization.