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. 2021 May 3;12:654225. doi: 10.3389/fimmu.2021.654225

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Copyright © 2021 Khan, Almutairi, Ali, Salcedo, Stewart, Wang and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of NK cell-specific IL-18 receptor-deficient mice. Excision of Il18r1 allele occurs predominantly in NK cells of NKp46-Cre-Il18r1^fl ^/ ^fl mice. (A) Protein expression of the IL-18 receptor alpha (IL-18Rα) on different subsets of splenic leukocytes from indicated mice. (B) NK cells from spleens of naïve Il18r1^fl ^/ ^fl (Cre-) and NKp46-Cre-Il18r1^fl ^/ ^fl (Cre+) littermate mice were enriched and stimulated with IL-18 ex vivo for 24 hours. 100 U/ml of rhIL-2 was added to maintain NK cell survival. Representative plots depict the mean fluorescence intensity (MFI) of CD71 and CD98 expression on cytokine-stimulated NK cells. The MFI expression is presented in percentage relative to the MFI of unstimulated cells as 100. Data are from one experiment representative of 3 independent experiments, with at least two replicates per group. Data represent mean + SD. NS, non-significant; ***p < 0.001.