| Reagent | Volume (μL) | Final concentration |
|---|---|---|
| 5× KAPA HiFi buffer | 10 | 1× |
| dNTPs (10 mM total) | 1.5 | 0.3 mM |
| Universal i5 index dilution | 5 | 1.5 mM |
| KAPA HotStart HiFi DNA polymerase (1 U/μL) | 1 | 0.2 U/μL |
| Master mix final volume | 17.5 | — |
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