Table 2 |.
uliCUT&RUN sample sequencing statistics
| Sample name | Target | Total reads | mm10 mapped reads | % Reads mm10 mapped | sacCer3 mapped reads | % Reads SacCer3 mapped |
|---|---|---|---|---|---|---|
| NoAb_500,000cells | No-antibody control | 12,519,390 | 7,724,115 | 61.69721528 | 4,386 | 0.035033656 |
| NoAb_50,000cells | No-antibody control | 21,124,738 | 13,619,070 | 64.46976999 | 18,632 | 0.08819991 |
| NoAb_5,000cells | No-antibody control | 22,840,561 | 13,639,479 | 59.716042 | 111,161 | 0.486682442 |
| NoAb_500cells | No-antibody control | 6,721,902 | 2,738,302 | 40.73701164 | 88,437 | 1.315654409 |
| NoAb_50cells | No-antibody control | 31,441,140 | 9,944,171 | 31.62789581 | 348,488 | 1.108382202 |
| NoAb_10cells | No-antibody control | 22,386,707 | 1,528,715 | 6.828672926 | 109,941 | 0.491099473 |
| CTCF_500,000cells | CTCF | 24,622,812 | 16,910,022 | 68.67624218 | 3,001 | 0.012187885 |
| CTCF_50,000cells | CTCF | 28,244,629 | 19,181,790 | 67.91305349 | 9,666 | 0.034222436 |
| CTCF_5,000cells | CTCF | 86,425,016 | 55,116,921 | 63.77426763 | 158,771 | 0.183709541 |
| CTCF_500cells | CTCF | 16,011,197 | 7,590,239 | 47.40581857 | 51,441 | 0.321281413 |
| CTCF_50cells | CTCF | 33,064,993 | 18,592,267 | 56.22945996 | 153,859 | 0.465322947 |
| CTCF_10cells | CTCF | 52,838,179 | 12,155,437 | 23.00502635 | 219,423 | 0.415273585 |
| NoAb_50cells | No-antibody control | 26,177,953 | 3,759,352 | 14.36075616 | 186,217 | 0.711350501 |
| Sox2_50cells | Sox2 | 37,306,970 | 3,788,443 | 10.15478608 | 140,045 | 0.375385618 |
| Nanog_50cells | Nanog | 11,878,529 | 4,291,975 | 36.13220963 | 58,996 | 0.496660823 |
| Oct4_50cells | Oct4 | 54,221,820 | 5,211,784 | 9.611968023 | 169,106 | 0.311878133 |
| NoAb_1cell_rep1 | No-antibody control | 23,265,110 | 74,478 | 0.320127435 | 51,691 | 0.222182487 |
| NoAb_1cell_rep2 | No-antibody control | 27,236,861 | 97,674 | 0.358609606 | 67,396 | 0.24744408 |
| NoAb_1cell_rep3 | No-antibody control | 21,219,173 | 50,829 | 0.239542795 | 43,526 | 0.205125808 |
| NoAb_1cell_rep4 | No-antibody control | 21,366,345 | 226,075 | 1.058089252 | 54,499 | 0.255069363 |
| NoAb_1cell_rep5 | No-antibody control | 64,767,963 | 196,799 | 0.303852385 | 134,532 | 0.207713804 |
| CTCF_1cell_rep1 | CTCF | 8,058,422 | 33,946 | 0.421248726 | 3,158 | 0.039188814 |
| CTCF_1cell_rep2 | CTCF | 29,785,051 | 117,613 | 0.394872582 | 71,249 | 0.239210603 |
| CTCF_1cell_rep3 | CTCF | 39,235,948 | 157,304 | 0.400918056 | 94,497 | 0.240842913 |
| CTCF_1cell_rep4 | CTCF | 25,623,725 | 167,324 | 0.653004198 | 63,394 | 0.247403529 |
| CTCF_1cell_rep5 | CTCF | 27,905,711 | 97,412 | 0.349075499 | 53,672 | 0.19233339 |
| blast_NoAb_rep1 | No-antibody control | 48,402,426 | 134,232 | 0.277324942 | 391,250 | 0.808327252 |
| blast_NoAb_rep2 | No-antibody control | 10,924,378 | 261,010 | 2.38924358 | 130,484 | 1.194429559 |
| blast_CTCF_rep1 | CTCF | 74,269,402 | 556,739 | 0.749620954 | 770,472 | 1.037401648 |
| blast_CTCF_rep2 | CTCF | 81,540,028 | 468,019 | 0.573974539 | 111,8201 | 1.371352239 |
Sample name: the name of the sample that describes the target, the number of cells (1–500,000), or if a blastocyst (blast) was assayed, and the replicate number, if applicable. Target: identity of the targeted chromatin-associated protein Total reads: the total raw sequencing reads for that sample mm10 mapped reads: the number of reads mapped to mm10 reference genome % Reads mm10 mapped: the percentage of total raw reads mapped to the mm10 reference genome sacCer3 mapped reads: the number of reads mapped to sacCer3 reference genome % sacCer3 mapped: the percentage of total raw reads mapped to sacCer3 reference genome