FIGURE 4.

Whole genome amplification and whole-exome sequencing of the putative DTC population. A, Map showing somatic single nucleotide polymorphisms (SNPs) or small structural variants (SVs), detected in each experimental sample but not CD45⁺ cells from the same patient as an internal control. Sample types are annotated as follows: gray, PC3 PCa cell line; orange, metastatic PCa patient; yellow, three localized PCa patients. Detected alterations are indicated by: white, no alteration detected; blue, SNP detected; red, SV detected; purple, SNP and SV both detected. All genes were selected from a list of 75 genes previously reported to be altered in prostate cancer. B, Allele frequencies for SNPs detected in the PCa putative DTC samples and PC3 cells. The median allele frequency for each sample is listed in the bottom row. DTC, disseminated tumor cell; PCa, prostate cancer