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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Prostate. 2019 Aug 26;79(14):1715–1727. doi: 10.1002/pros.23896

FIGURE 5.

FIGURE 5

Identity of contaminating cell types. A, Estimation of the number of contaminating myeloid precursors by colony formation. Upper left; example images of each colony type. Upper right: Example FACS plots describing the cell populations examined. Data are a representative experiment mean ± SD of the six rows of a 24-well plate. Means were compared by the Student t test. B, Estimation of contaminating cell types by morphology. Top: Hema3 (Wright-Giemsa) stained smears prepared after sorting the indicated cell populations; bottom: 500 cell marrow differential cell counts were performed by a blinded observer of smears from five patients. Data represent mean ± SEM. C, Estimation of immune cell content in the putative DTC population from the RNA-Seq data (as in Figure 3) using the CIBERSORT algorithm. FACS, fluorescence-activated cell sorting