TABLE 2.
Results summary. Left column “negative” and “positive” denote either less than four putative DTCs per million BM cells or more than four putative DTCs per million BM cells respectively. “RNA-Seq positive” denotes increased expression of PCa marker genes on RNA sequencing of putative DTCs. “WGA/WES positive” denotes the presence of mutations in PCa genes after whole genome amplification and whole-exome sequencing
| Clinical/FACS category | Total | DTCs collected | RNA-Seq positive | RNA-Seq negative | WGA/WES positive | WGA/WES negative | qRT-PCR positive | qRT-PCR negative or equivocal |
|---|---|---|---|---|---|---|---|---|
| Normal donor DTC negative | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
| Normal donor DTC positive | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
| Localized PCa DTC negative | 48 | 8 | 3 | 2 | 1 | 1 | 11 | 13 |
| Localized PCa DTC positive | 10 | 9 | 1 | 2 | 1 | 0 | 1 | 5 |
| Localized PCa no FACS data | 14 | 0 | 0 | 0 | 0 | 0 | 10 | 4 |
| Metastatic PCa DTC negative | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Metastatic PCa DTC positive | 4 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Abbreviations: BM, bone marrow; DTCs, disseminated tumor cells; FACS, fluorescence-activated cell sorting; PCa, prostate cancer; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction.