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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2021 Apr 22;1867(8):166153. doi: 10.1016/j.bbadis.2021.166153

Fig. 1.

Fig. 1.

Effect of different isoBAs and corresponding stereoisomers on farnesoid X receptor (FXR) activation. (A) mRNA expression levels and (B) pre-mRNA levels of FXR target genes (Ostb, Kng1) in HepG2 cells treated for 6 h with 3-alpha hydroxy and corresponding isoBAs. (C) Effect of the synthetic FXR ligand (GW4064) on mRNA and pre-mRNA levels of Ostb, Kng1, Shp. (D) mRNA half-life of FXR target genes and (E) distribution of Fxr and its target gene mRNAs (cytosol vs. nucleus); n = 3–4, of at least two independent experiments. Values represent the mean ± SEM. *p < 0.05, #p < 0.01.