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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2021 Apr 22;1867(8):166153. doi: 10.1016/j.bbadis.2021.166153

Fig. 3.

Fig. 3.

Measurement of FXR activation by isoBAs in real-time using a bile acid sensor (NucleoBAS). (A) Changes in FRET emission ratio upon addition of 100 μM isoCDCA or CDCA at t = 120 s and 5 μM GW4064 at t = 240 s. (B) Changes in FRET emission ratio upon addition of 5 μM GW4064 alone at t = 120 s and at t = 240 s. (C) Changes in FRET emission ratio upon addition of 100 μM isoDCA or DCA at t = 120 s and 5 μM GW4064 at t = 240 s. (n = 6 cells per experiment). At least three independent experiments were performed; Error bars represent SEM.