Skip to main content
. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Science. 2021 May 14;372(6543):716–721. doi: 10.1126/science.aaz2740

Fig. 1. Chemical suppressor screen for the mon zebrafish mutant identifies DHODH inhibitors to rescue primitive erythropoiesis.

Fig. 1.

(A) Cartoon depicting chemical screening strategy. WT, wild type; het, mon heterozygous. (B) WISH for βe3 globin in mon and WT or het zebrafish embryos at 22 hpf treated with 7 μM leflunomide or DMSO. N (number of biological replicates) = 4. Cumulative results are shown. (C) Benzidine staining of embryos at 44 hpf after morpholino-mediated knockdown of tif1γ (tif1γ KD) or standard control knockdown (Ctrl KD) and treatment with 3.5 μM brequinar or DMSO from 5.3 hpf until 11.7 hpf. N = 3. Data from one representative experiment are shown. n = number of embryos with depicted phenotype/number of all embryos tested (see also fig. S1).