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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: ACS Chem Biol. 2019 Oct 21;14(12):2564–2575. doi: 10.1021/acschembio.9b00365

Figure 2.

Figure 2.

Representative denaturing PAGE images of 100-mer duplexes containing DPCs or DpCs. (A) DNA-protein cross-links conjugated to the C5 of dC. Lanes 1: starting DNA (100-mer duplex containing 5fC); lanes 2 and 3: gel purified 5fC-mediated histone H4 DPCs located on transcribed strands (TS) and non-transcribed strands (NTS). (B) DNA-protein cross-links conjugated to C7 of 7-deazaguanine. Lane 4: starting DNA (100-mer duplex containing 7-deaza-DHP-dG); Lane 5 and 6: gel purified 7-deazaG-mediated histone H2A and AGT DPCs located on transcribed strands (TS). (C) DNA-peptide cross-links at C7 of 7-deazaguanine containing 31-mer peptide. Lane 7: starting DNA (100mer DNA duplex containing 7-deaza-DHP-dG); lane 8: gel purified DpC conjugate located on transcribed strands (TS); lane 9: protease K digested DNA-peptide conjugates.