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. Author manuscript; available in PMC: 2022 Jan 18.
Published in final edited form as: Chem Res Toxicol. 2020 Dec 31;34(1):119–131. doi: 10.1021/acs.chemrestox.0c00376

Figure 3.

Figure 3.

Micronuclei measurement in HapMap cell lines upon DEB treatment: Eighteen lymphoblastoid cells were exposed to 0, 5 and 10 μM DEB for 6 h in triplicate. The cells were collected by centrifugation and then incubated in fresh media for an additional 96 h. The number of MN were determined with a Litron Microflow micronucleus analysis kit coupled with flow cytometry. Representative flow cytometry plots are displayed in Figure S7. A. Percentage of MN in 18 HapMap cell lines after DEB treatment. B. Comparison of MN formation in GSTT1 negative and positive cell lines (mean ± SD). DEB significantly increased the number of MN (p < 0.05 by Welch’s t-test and p < 0.001 by Anova analysis). GSTT1 genotype had no effect on the number of DEB-induced MN. Cell lines are color coded based on GSTT1 genotype, null (orange), heterozygote (light blue) and homozygote (dark blue), and organized based on DEB-induced apoptosis after 10 μM treatment (Figure 2). Gray bars indicate control cell lines and light and dark shades of orange or blue indicate 5 or 10 μM DEB treatment, respectively.