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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: DNA Repair (Amst). 2020 Mar 19;89:102840. doi: 10.1016/j.dnarep.2020.102840

Figure 1.

Figure 1.

Detection of cisplatin-induced DNA-protein cross-links by gel electrophoresis. 12% SDS-PAGE analysis of 32P-endlabeled DNA duplexes (5'-GGA GCT GGT GGC GTA GGC-3’ +strand) following incubation with (A) recombinant human AGT or (B) recombinant GAPDH protein in the presence of 1 (lane 4), 5 (lane 5), 10 (lane 6), 50 (lane 7) or 100 (lane 8) molar equivalents of cisplatin. Free duplex DNA (labeled “Free oligo”) migrates to the bottom of the gel, whereas DNA-protein cross-links display substantially reduced mobility.