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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: DNA Repair (Amst). 2020 Mar 19;89:102840. doi: 10.1016/j.dnarep.2020.102840

Figure 3.

Figure 3.

HPLC-ESI+-MS and deconvoluted spectra (inset) of (A) unreacted AGT protein, (B) dG-Pt-Cl monoadduct-treated AGT protein that had been separated by HPLC with retention time 32.1 min in Figure 2, and (C) dG-Pt-Cl monoadduct-treated AGT protein that had been separated by HPLC with retention time 30.1 min in Figure 2. Unmodified AGT (calculated, M = 21876 Da; observed, M = 21878 Da), AGT containing a single platinum cross-link to dG (calculated, M = 22354 Da; observed, M = 22355 Da or calculated, M = 22371 Da; observed, M = 22372 Da), AGT containing two platinum cross-links to dG (calculated, M = 22832 Da; observed, M = 22832 Da), and AGT containing three platinum cross-links to dG (calculated, M = 23327 Da; observed, M = 23328 Da).