. Author manuscript; available in PMC: 2021 Jun 4.

Published in final edited form as: DNA Repair (Amst). 2020 Mar 19;89:102840. doi: 10.1016/j.dnarep.2020.102840

Table 2.

Platinated peptide sequences of AGT after treatment with dG-Pt-Cl followed by HPLC-ESI⁺-MS/MS analysis of the resulting tryptic digests. The assigned platinum binding site is given as bold italic type within peptide sequence.

Peptide sequence	Pt adduct	Pt adduct mass (Da)	Charge	SEQUEST parameters		Ions^*	MS/MS spectrum
Peptide sequence	Pt adduct	Pt adduct mass (Da)	Charge	Xcorr	ΔCn	Ions^*	MS/MS spectrum

F¹⁰⁸GEVISYQQLAALAGNPK¹²⁵	+(Pt-dG-2NH₃)	495.11	+3	4.53	1.0	34/68	Figure 2.4A
F¹⁰⁸GEVISYQQLAALAGNPKAAR¹²⁸	+(Pt-Gua)	344.00	+3	3.39	1.0	28/60	Figure 2.4B
G¹³⁶NPVPILIPCHR¹⁴⁷	+(Pt-Gua)	344.00	+2	2.54	1.0	22/44	Figure 2.4C
G¹³⁶NPVPILIPCHR¹⁴⁷	+(Pt-Gua-NH₃)	361.03	+2	3.07	1.0	20/50	Figure 2.4D
G¹³⁶NPVPILIPCHR¹⁴⁷	+(Pt-dG-2NH₃)	495.11	+2	3.05	1.0	16/35	Figure 2.4E
V¹⁴⁸VCSSGAVGNYSGGLAVK¹⁶⁵	+(Pt-Gua-2NH₃)	378.05	+3	4.95	1.0	30/68	Figure 2.4F

Ratio of the assigned b and y ions to the total number of all possible fragment ions.