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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Mol Microbiol. 2020 Aug 10;114(4):681–693. doi: 10.1111/mmi.14576

Fig. 3. Creation of mutant HdrR proteins with defects in transcription activation.

Fig. 3.

A) Illustration of the alanine scanning mutagenesis scheme. Blocks of 6 consecutive alanine mutations were engineered throughout the entire length of HdrR for a total of 22 separate mutants. The mutants were named sequentially starting with Ala1, which contained alanine substitutions in amino acids #2 – 7. B) Illustration of the luciferase screening procedure to identify mutant proteins defective in hdrRM operon transcription activation. C) Each of the 22 alanine mutant hdrR genes was ectopically expressed to assess its ability to activate gene expression from a reporter strain containing a luciferase ORF replacement of the hdrRM ORFs. Data are presented relative to the parent reporter strain (Basal), which was arbitrarily assigned a value of 1. The results represent the means of three independent experiments ± SD.