Fig 2. Demarcated areas for contamination by the test microorganisms for evaluation of the potential of instantaneous disinfection by the spray biocidal agent (0.25% sodium hypochlorite).

The red circles represent the inoculation area (30 cm²) used as control (right side), the blue circles represent the inoculation area (30 cm²) used as test (left side), and the numbers in the blue circles represent the positions of the water-sensitive papers for each experiment: (1) cap; (2) safety glasses; (3) respirator face mask; (4) lab coat; (5) glove; and (6) shoe. For the control, the surface of the items was swabbed for microorganism collection immediately after surface contamination, while for the tests, the surfaces were swabbed after predetermined exposure times to the biocidal agent.