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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Biochemistry. 2020 Jan 2;59(4):563–581. doi: 10.1021/acs.biochem.9b00943

Table 1.

Binding Affinities and Binding Energetics for Nrf234–100 Alanine Variants

Nrf2 WT D77A E78A E79A T80A E82A F83A
KD (nM) 10±2 (4.4 ±1.8) ×103 25 ± 3 (1.7 ± 0.2) ×103 (2.6 ± 1.5) ×104 (2.2±1.3) ×104 18 ± 3
ΔΔG (kcal/mol) n/a 3.5 ± 0.3 0.5 ± 0.1 3.0 ± 0.1 4.5 ± 0.4 4.4 ± 0.4 0.3 ± 0.1