Skip to main content
. 2021 Jun 4;16(6):e0251604. doi: 10.1371/journal.pone.0251604

Fig 1. Colon tissue Per2::Luc mice Per2 protein displays typical interaction with Cry2 and Bmal1 circadian proteins.

Fig 1

We compared interactions of Per2::Luc fusion protein vs. WTBL6 Per2 protein with well validated circadian protein binding partners for Per2, namely Cry2 and Bmal1 proteins using immunoprecipitation with Per2 Ab of colon tissue lysates. Data for Cry2 (left) and Bmal1 (right) show no significant difference in binding of either Cry2 or Bmal1 with the Per2::Luc protein vs. the normal BG proteins. The relative density of Cry2 and Bmal1 are represented for BL6 and Per2::Luc mice. There was no difference in colon tissue levels of Cry2 or Bmal1 levels at a single time point (p = 1.0 and 0.7, respectively). Blots are representative of n = 4 mice.