Fig 2. Intra-islet COL18 core protein colocalizes with insulin not glucagon staining in wt pancreas and is progressively lost in db/db pancreas.

Immunofluorescence staining of (A, F, K,) COL18, (B, G, L) insulin (INS), (C, H, M) insulin and COL18 merged, (D, I, N) glucagon (GLUC) and COL18 merged in 9 wk wt (A-D), 9 wk db/db (F-I) and 4 wk db/db (K-N) pancreas. (E, J, O) Nuclei were stained with DAPI. Scale bar = 20 μm.