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. 2021 Jun 4;16(6):e0241532. doi: 10.1371/journal.pone.0241532

Table 1. Facial expressions during the interventions as defined by the HFI method.

Eye white increase (AD1) Nostril dilator (AD38) Inner brow raiser (AU101) Blink (AU145) Half blink (AU47) Upper lid raiser (AU5) “Ear flicker” Ear rotator (EAD104)
Transportation
Percentage of AUs during intervention / control 8.2% / 4.8% 13.1% / 8.4% 5.3% / 8.1% 12.7% / 19.6% 7.7% / 11.2% 8.0% / 5.7% 18.9 / 17.7% Not selected
Difference in frequency 113.7% 106.2% 31.4% 30.1% 35.8% 98.6% 76.2% Not selected
Social isolation
Percentage of AUs during intervention / control 7.8% / 3.9% 15.0% / 7.0% 15.0% / 12.1% 18.1% / 19.8% Not selected Not selected 16.6% / 20.2% 5.3% / 6.2%
Difference in frequency 85.7% 90.9% 43.0% 12.8% Not selected Not selected 1.9% 6.1%
Combined
Percentage of AUs during intervention / control 8.2% / 4.8% 13.4% / 8.4% 7.2% / 8.1% 13.8% / 19.6% 8.0% / 11.2% 7.0% / 5.7% 18.4% /17.7% Not selected
Difference in frequency 106.8% 101.8% 52.1% 29.3% 30.5% 80.7% 66.4% Not selected

Action units (AUs) and action descriptors (ADs) selected using the Human FACS Investigation (HFI) method to represent stressful interventions in horses in the transportation and social isolation groups and together as a combined group.