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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Biochemistry. 2020 Jun 15;59(25):2340–2350. doi: 10.1021/acs.biochem.0c00257

Figure 1.

Figure 1.

Sequence-based distribution of HD-GYP proteins, homology model of VCA0681, and hydrolytic activities of two HD-[HD-GYP] proteins with c-di-GMP and 3′3′ c-GAMP. (A) Cluster of the SSN containing only the HD-[HD-GYP] proteins (187 sequences). (B) Homology model of VCA0681. The two diiron cofactors have been modeled and are shown as orange spheres. The histidine and aspartate ligands coordinating the metal ions are colored black and green, respectively. (C) c-di-GMP hydrolysis by the diiron WT SO3491 and MBP-VCA0681 in the dithionite-reduced form. Experimental conditions: 1 μM diFe and 150 μM c-di-GMP. (D) 3′3′ c-GAMP hydrolysis by the diiron WT SO3491 and MBP-VCA0681 in the dithionite-reduced form. Experimental conditions: 5 μM diFe, 150 μM c-di-GMP, and 200 μM dithionite. Experiments were performed under O2-free conditions. Similar results were obtained for different protein preparations.