Skip to main content
. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Biochemistry. 2020 Jun 15;59(25):2340–2350. doi: 10.1021/acs.biochem.0c00257

Figure 2.

Figure 2.

Mössbauer spectra and time-dependent c-di-GMP hydrolysis by the 57Fe-labeled WT MBP-SO3491 in different redox states. (A) 80 K Mössbauer spectrum of WT MBP-SO3491 reduced with 5 mM ascorbate. (B) 80 K Mössbauer spectrum of WT MBP-SO3491 reduced with 10 mM dithionite. The amount of Fe2II is shown with the red trace (experimental spectrum), and the amount of FeIIFeIII with the blue trace (simulation). (C) c-di-GMP hydrolysis by ascorbate- and dithionite-reduced 57Fe-labeled WT MBP-SO3491. The error bars represent the standard deviation.