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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Biochemistry. 2020 Jun 15;59(25):2340–2350. doi: 10.1021/acs.biochem.0c00257

Figure 3.

Figure 3.

HPLC chromatograms monitoring the reaction of WT MBP-SO3491 with different nucleotides. The x-axis is the elution time, and the y-axis is the absorbance at 254 nm. All reactions were performed with 5 μM protein (diiron concentration) and 50 μM substrate analogues under O2-free conditions. The trace label corresponds to the substrate nucleotide. p1 corresponds to 5′-pGpG, p2 to 5′-pApG,38 p3 to 3′-AMP, p4 to 2′-AMP, and p5 to 3′-GMP.