Figure 1.

Rs4142441‐MUT is associated with decreased expression of long non‐coding RNA (lncRNA) OSER1‐AS1. A, pGL3 firefly luciferase reporter plasmids with wild‐type or mutant allele for rs4142441. Rs4142441‐WT represents the sequence with the wild‐type allele (A) of rs4142441. Rs4142441‐MUT represents the sequence with the mutant allele (G) of rs4142441. B, The pGL3 firefly luciferase reporter plasmids with wild‐type or mutant allele for rs4142441 were transiently transfected into H1299 cells with a Renilla luciferase reporter for normalization. Luciferase activities were measured after 48 h. Data are presented as the means and standard deviations (SD) of separate transfections (n = 3). Error bars represent the SD. **Mann‐Whitney U test, P < .01. C, The pcDNA3.1 plasmid with its CMV promoter replaced by the promoter region of OSER1‐AS1 (± 1000 bp on both sides of rs4142441). Rs4142441‐WT represents the sequence with the wild‐type allele (A) of rs4142441. Rs4142441‐MUT represents the sequence with the mutant allele (G) of rs4142441. The cDNA sequence of OSER1‐AS1 (transcript ID: ENST00000442383.1) was inserted into the multiple cloning site. D, OSER1‐AS1 expression levels were measured after 48 h of transfection. The relative normalized expression level was calculated as the fold change of the expression level of rs4142441‐WT(A) vector relative to rs4142441‐MUT(G), normalized to the expression level of β‐actin. Data are presented as the means and SD of separate transfections (n = 3). Error bars represent the SD. **Mann‐Whitney U test, P < .01