Figure 6.

Influence of OSER1‐AS1 on the expression levels of MYC, BCL2L11 and miR‐17‐5p in H1299 cells. A, Protein expression levels of MYC and BCL2L11 in H1299 cells were detected by western blot after knockdown and overexpression of OSER1‐AS1. Cell lysates were collected for western blotting 48 h post‐transfection. Numbers below the bands indicate the fold change of quantitative analysis results. Beta‐actin was used as positive control for cytoplasmic cell lysate. Histone H3 was used as positive control for nuclear cell lysate. B, MiR‐17‐5p expression levels in nucleus and cytoplasmic cell lysate after overexpression or knockdown of OSER1‐AS1. C, BCL2L11 expression levels in nuclear and cytoplasmic cell lysate after overexpression or knockdown of OSER1‐AS1. Error bars represent the SD of three independent experiments. Two‐tailed Student’s t test: *P < .05; **P < .01