FIGURE 3.

Treatment of HL cell lines with hydrogen peroxide induces large mono‐ and multinuclear cells. A, Cellular morphology of HL cell lines (KMH2 and L428) and a non‐HL cell line (Namalwa) treated with the indicated concentrations of hydrogen peroxide for 72 h. Scale bar, 20 μm. B, Flow cytometric analyses of changes in cell size of HL cell lines treated with hydrogen peroxide for 48 h. The vertical axis indicates MFI of forward‐scattered light (left). Ploidy analysis of HL cell lines treated with hydrogen peroxide for 48 h; the vertical axis indicates the ≧ 4N cells (cells with DNA content of tetraploid or more) rate from PI‐negative total live cells (right). *P < .05. C, HL cell lines retain resistance to cytotoxic effects by hydrogen peroxide. HL cell lines (KMH2 and L428) and non‐HL cell lines (Namalwa, BJAB, Ramos, and Jurkat) were treated with hydrogen peroxide for 48 h, and the expression of Annexin V was detected by flow cytometry. *P < .05