FIGURE 3.

LINC01123 enhanced cell proliferation by modulating Gli1 expression. A, Overexpression efficacy of Gli1 in sh‐ LINC01123 OS cells (U‐2OS and 143B) was detected by western blotting. B and C, Overexpression of Gli1 partly reversed the suppressed effects of LINC01123‐knockdown on the cell viability of U‐2OS and 143B cells using the CCK‐8 assay. Data were presented as the mean ±SD, *P <.05, **P <.01. D and E, Overexpression of Gli1 partly reversed the suppressed effects of LINC01123‐knockdown on the cell viability of U‐2OS and 143B cells using the colony formation assay. Data were presented as the mean ±SD, **P <.01, ***P <.001. F‐I, Overexpression of Gli1 partly reversed the suppressed effects of LINC01123‐knockdown on the Cell migration (F and G) and invasion (H and I) ability of U‐2OS and 143B cells. Scale bars =50 μm. Data were presented as the mean ±SD, **P <.01, ***P <.001. J and K, Overexpression of Gli1 partly reversed the inhibitory effects of LINC01123‐knockdown on the self‐renewal ability of U‐2OS and 143B cells. Scale bars =50 μm. Data were presented as the mean ±SD, ***P <.001