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. 2021 May 7;112(6):2260–2271. doi: 10.1111/cas.14913

FIGURE 5.

FIGURE 5

LINC01123 bound to miR‐561b‐5p and decreased its expression in the OS cells. A, miR‐561b‐5p expression was detected in OS cells with LINC01123 knockdown or not. B, The wild‐type and the mutated sequences of the LINC01123 mRNA 3’‐UTR (mutation site: red). C, Top panel shows a schematic image of a construction containing LINC01123 wild type combined with MS2 binding sequence. MS2‐RIP followed by miR‐561b‐5p qRT‐PCR to measure miR‐561b‐5p endogenously associated with LINC01123. Data were presented as the mean ±SD, **P <.01. D, U‐2OS and 143B cells lysate were incubated with biotin‐labeled LINC01123, qRT‐PCR measured miR‐561b‐5p expression in the products of pulldown by biotin, Data were presented as the mean ±SD, **P <.01. E and F, The relative luciferase activity of luciferase reporters containing LINC01123 wild‐type (WT) or mutated (MUT) co‐transfected with miR‐561b‐5p or its negative control in U‐2OS and 143B cells was assessed. Data were presented as the mean ±SD, **P <.01. G and H, AGO2‐RIP followed by qRT‐PCR to evaluate LINC01123 level after miR‐561b‐5p overexpression. **P <.01, ***P <.001