FIGURE 4.

The influence of CDKN1C expression on mantle cell lymphoma (MCL) cell proliferation. A, Western blotting confirmation of loss of CDKN1C in the CRISPR/Cas9 CDKN1C knockout of JeKo‐1. Lysates were obtained from control (WT) or CDKN1C‐knockout (KO) JeKo‐1 that had been exposed to 300 nmol/L OR‐S1 for 5 d. β‐actin was used as a loading control. B, Partial acquisition of resistance to OR‐S1 in CDKN1C‐knockout JeKo‐1. Control (WT) and CDKN1C‐knockout (KO) JeKo‐1 were treated with vehicle or several concentrations of OR‐S1 (30, 100, and 300 nmol/L) for 4 d. Data are shown as relative cell viability (the mean of triplicates ± SD) compared with each vehicle control defined as 1. *P < .05, **P < .01. C, Inhibitory effect of enforced CDKN1C expression on REC‐1 cell proliferation. CDKN1C was overexpressed in REC‐1 using retroviral transduction. The stable transfectant was cultured until day 5 (counted on day 2 and day 5). Data represent the mean of triplicates ± SD. *P < .05