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. 2021 Apr 7;112(6):2210–2222. doi: 10.1111/cas.14900

FIGURE 3.

FIGURE 3

MZT2A regulation of Akt phosphorylation in NSCLC. A, Western blot. NSCLC A549 and H1299 cells were grown, transfected with MZT2A cDNA or siRNA, and subjected to western blot analysis. Graphs show the quantified data. B, RT‐qPCR. NSCLC A549 and H1299 cells were grown, transfected with MZT2A cDNA or siRNA, and subjected to RT‐qPCR. C, Western blot. A549 and H1299 cells were grown, transfected with MZT2A cDNA, treated with or without the selective Akt inhibitor LY294002, and subjected to western blot analysis. Graphs show the quantified data. D, Cell viability MTT assay: A549 and H1299 cells were grown, transfected with MZT2A cDNA, treated with or without the selective Akt inhibitor LY294002, and subjected to MTT assays. E, Transwell assay. A549 and H1299 cells were grown, transfected with MZT2A cDNA, treated with or without the selective Akt inhibitor LY294002, and subjected to Transwell assays. Graphs show the quantified data