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. 2021 Apr 7;112(6):2210–2222. doi: 10.1111/cas.14900

FIGURE 5.

FIGURE 5

The MZT2A functional domain MOZART2 mediates MZT2A functions in the regulation of Akt phosphorylation. A, Illustration of our creation of 2 mutated MZT2A MOZART2 domain constructs. ARS: antibody recognition sequences B, Western blot. A549 and H1299 cells were grown and transfected with the 2 mutated MZT2A cDNA constructs, and then were subjected to western blot analysis. C, D, Co‐IP‐western blot. A549 and H1299 cells were grown, transfected with the 2 mutated MZT2A cDNA, and subjected to Co‐IP‐western blot. E, Western blot. A549 and H1299 cells were grown, transfected with the 2 mutated MZT2A cDNAs, and subjected to Co‐IP‐western blot analysis. F, Transwell assay. A549 and H1299 cells were grown, transfected with the 2 mutated MZT2A cDNAs, and subjected to Transwell assays. Graphs show the quantified data. G, Cell viability MTT assay. A549 and H1299 cells were grown, transfected with the 2 mutated MZT2A cDNAs, and subjected to MTT assays