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. 2021 May 21;12:648709. doi: 10.3389/fgene.2021.648709

FIGURE 4.

FIGURE 4

Circ_0136474 could target miR-766-3p in human chondrocytes. (A) Schematic diagram showed the construction of pGL3 luciferase report vectors carrying WT-circ_0136474 or MUT-circ_0136474. (B) qPCR detected the relative expression of miR-766-3p in CHON-001 cells transfected with miR-766-3p/NC mimic (miR-766-3p/NC). (C) The dual-luciferase reporter assay measured the relative luciferase activity of WT-circ_0136474 and MUT-circ_0136474 vectors with co-transfection of miR-766-3p/NC. (D) The radioimmunoprecipitation assay (RIP) assay confirmed the relative RNA enrichments of circ_0136474 and miR-766-3p in CHON-001 cells. (E–H) qPCR detected the relative miR-766-3p expression in (E) OA cartilages (n = 33) and normal cartilages (n = 33), (F) control CHON-001 cells and IL-1β (10 ng/m-)-induced CHON-001 cells, (G,H) IL-1β (10 ng/ml)-induced CHON-001 cells pre-transfected with anti-miR-766-3p/NC, si-circ_0136474/NC, or si-circ_0136474 combined with anti-miR-766-3p/NC. *P < 0.05 and **P < 0.01 were determined by unpaired t-test, ordinary one-way ANOVA, or two-way ANOVA.