FIGURE 7.

Restoring miR-766-3p attenuated IL-1β-induced oxidative injury in chondrocytes, and this effect was abrogated by DNMT3A upregulation. CHON-001 cells were transfected with miR-766-3p/NC or miR-766-3p combined with DNMT3A or vector prior to treatment of IL-1β (10 ng/ml) for 24 h. (A) CCK-8 monitored the OD values of inoculated cells during 72 h. (B) FCM tested the apoptosis rate (%). (C) Western blotting examined the relative protein expression of PCNA, Bcl-2, and Bax. (D–G) Special assay kits determined the levels of ROS, GSH, MDA, and SOD. *P < 0.05 was determined by ordinary one-way ANOVA or two-way ANOVA.