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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2021 Apr 17;530:111286. doi: 10.1016/j.mce.2021.111286

Fig. 1.

Fig. 1.

Androgen status regulates Gnrhr mRNA in vivo. (A) Wildtype male mice received sham or castration (Cx) surgery, and pituitaries were collected 1 week later for analysis of Gnrhr mRNA by qRT-PCR. Data are normalized to Gapdh and represented as mean fold change relative to sham ± SEM. n = 6 animals per group. **, p < 0.01 by Student t-test. (B) Wildtype male mice were Cx with or without physiological DHT replacement, and pituitaries were collected 1 week later. Data are normalized to Ppia and represented as fold change relative to Cx ± SEM. n = 5–6 animals per group. *, p < 0.05 by Student t-test. (C) 6-month old female mice were ovariectomized (Ovx) with or without DHT and pituitaries were collected 1 week later for analysis of Gnrhr mRNA by qRT-PCR. Data are normalized to Gapdh and represented as mean fold change relative to Ovx ± SEM. n = 5–6 animals per group. *, p < 0.05 by Student t-test.