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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2021 Apr 17;530:111286. doi: 10.1016/j.mce.2021.111286

Fig. 2.

Fig. 2.

GnRHR-luciferase is positively regulated by androgens. LβT2 cells were transiently transfected with rAR (AR+) or empty vector (AR−) and the mouse −600 bp GnRHR-luciferase reporter. Cells were treated with vehicle control or (A) R1881, (B) DHT, or (C) T at concentrations ranging from 10−9 M to 10−5 M for 24 h before harvest for luciferase and β-galactosidase assays. For each hormone treatment, data are represented as mean fold change relative to AR+/ vehicle ± SEM for three independent experiments performed in triplicate. *, p < 0.05; **, p < 0.01 by one-way ANOVA.