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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2021 Apr 17;530:111286. doi: 10.1016/j.mce.2021.111286

Fig. 7.

Fig. 7.

AR does not bind directly to HREs at −159/−144 or −499/−484 in electrophoretic mobility shift assays. Rat AR was in vitro transcribed and translated from a pSG5 expression vector, and incubated with the consensus HRE sequence, the −159/−144 HRE, or the −499/−484 HRE and tested for protein-DNA complex formation by EMSA. rAR bound the consensus HRE sequence (lane 1) and was supershifted with an AR antibody (lane 2) but not with control IgG (lane 3). rAR binding was not observed for either the −159/−144 HRE or the −499/−484 HRE. “Ab” indicates antibody and “comp” indicates cold competitor at 100-fold excess.