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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2021 Apr 17;530:111286. doi: 10.1016/j.mce.2021.111286

Fig. 9.

Fig. 9.

AR is recruited to the −159 HRE locus within the endogenous Gnrhr promoter in LβT2 cells. LβT2 cells were transiently transfected with rAR and then treated with 10−7 M R1881 for 24 h. Chromatin was collected and immunoprecipitated using Santa Cruz AR antibody (SC AR), Active Motif AR antibody (AM AR), or normal rabbit IgG (negative control). qPCR was performed using primers specifically targeting the −499 HRE or −159 HRE within the Gnrhr proximal promoter or a gene desert within mouse chromosome 14 (negative control). Data are represented as mean percent input ± SEM for three independent experiments performed in triplicate. Data were analyzed by two-way ANOVA with Tukey post hoc analysis. Stars indicate significant enrichment compared to IgG for each locus tested. *, p < 0.05; ****, p < 0.0001.