Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, Strain background (Mus musculus) | Gli1CreER | Jackson Laboratory | Stock No: 007913 | |
| Strain, Strain background (Mus musculus) | Rosa26 lsl-tdTomato | Jackson Laboratory | Stock No: 007909 | |
| Strain, Strain background (Mus musculus) | Rosa26 lsl-DTA | Jackson Laboratory | Stock No: 010527 | |
| Sequence-based reagent |
Gli1CreER
Primer 1 |
Jackson Laboratory | PCR Primer | 5’-GCGGTCTGGCAGTAAAAACTATC-3’ |
| Sequence-based reagent |
Gli1CreER
Primer 2 |
Jackson Laboratory | PCR Primer | 5’-GTGAAACAGCATTGCTGTCACTT-3’ |
| Sequence-based reagent |
Gli1CreER
Primer 3 |
Jackson Laboratory | PCR Primer | 5’-CACGTGGGCTCCAGCATT-3’ |
| Sequence-based reagent |
Gli1CreER
Primer 4 |
Jackson Laboratory | PCR Primer | 5’-TCACCAGTCATTTCTGCCTTTG-3’ |
| Sequence-based reagent |
Rosa26 lsl-tdTomato
Primer 1 |
Jackson Laboratory | PCR Primer | 5’-AAGGGAGCTGCAGTGGAGTA-3’ |
| Sequence-based reagent |
Rosa26 lsl-tdTomato
Primer 2 |
Jackson Laboratory | PCR Primer | 5’-CCGAAAATCTGTGGGAAGTC-3’ |
| Sequence-based reagent |
Rosa26 lsl-tdTomato
Primer 3 |
Jackson Laboratory | PCR Primer | 5’-GGCATTAAAGCAGCGTATCC-3’ |
| Sequence-based reagent |
Rosa26 lsl-tdTomato
Primer 4 |
Jackson Laboratory | PCR Primer | 5’-CTGTTCCTGTACGGCATGG-3’ |
| Sequence-based reagent |
Rosa26 lsl-DTA
Primer1 |
Jackson Laboratory | PCR Primer | 5’-CGACCTGCAGGTCCTCG-3’ |
| Sequence-based reagent |
Rosa26 lsl-DTA
Primer 2 |
Jackson Laboratory | PCR Primer | 5’-CCAAAGTCGCTCTGAGTTGTTATC-3’ |
| Sequence-based reagent |
Rosa26 lsl-DTA
Primer 3 |
Jackson Laboratory | PCR Primer | 5’-GAGCGGGAGAAATGGATATG-3’ |
| Sequence-based reagent |
Rosa26 lsl-DTA
Primer 4 |
Jackson Laboratory | PCR Primer | 5’-CTCGAGTTTGTCCAATTATGTCAC-3’ |
| Antibody | Rabbit polyclonal Anti-Gli1 | NOVUS biologicals | NB600-600 | IF (1:100) |
| Antibody | Rabbit polyclonal Anti-Ki67 | Abcam | ab15580 | IF (1:50) |
| Antibody | Rat monoclonal Anti-Sca1 | Santa Cruz Bio | sc-52601 | IF (1:200) |
| Antibody | Rat monoclonal Anti-Cd200 | Santa Cruz Bio | sc-53100 | IF (1:100) |
| Antibody | Mouse monoclonal Anti-Cd90 | Santa Cruz Bio | sc-53456 | IF (1:100) |
| Antibody | Mouse monoclonal Anti-PDGFRα | Santa Cruz Bio | sc-398206 | IF (1:200) |
| Antibody | Mouse monoclonal Anti-Cd248 | Santa Cruz Bio | sc-377221 | IF (1:200) |
| Antibody | Rabbit polyclonal anti-Prg4 | Abcam | ab28484 | IF (1:50) |
| Antibody | Rat monoclonal Anti-Sca1 | BioLegend | 108131 | Flow analysis (1:100) |
| Antibody | Mouse monoclonal Anti-Cd90 | BioLegend | 202526 | Flow analysis (1:100) |
| Antibody | Rat monoclonal Anti-Cd200 | BioLegend | 123809 | Flow analysis (1:100) |
| Antibody | Rat monoclonal Anti-PDGFRα | BioLegend | 135907 | Flow analysis (1:100) |
| Software, algorithm | Graphpad 8.0 | Statistical Analysis | Graph preparation, statistical analysis |