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. 2021 May 25;10:e65935. doi: 10.7554/eLife.65935

Figure 3. Avoidance of E. faecalis is independent of virulence.

(A) Occupancy index of N2 animals on virulent E. faecalis OG1RF and avirulent E. faecalis OG1RF ΔfsrB at 4 hr. An unpaired t-test was performed. (B) Quantification of anterior intestinal diameter of N2 animals on E. faecalis strain OG1RF or OG1RF ΔfsrB at 4 hr. An unpaired t-test was performed. (C) Representative fluorescent micrographs of clec-60p::GFP (top) and ilys-3p::GFP (bottom) animals at 0, 4, and 24 hr on E. faecalis. Scale bar, 200 µm. (D) Quantification of clec-60::GFP at 0, 4, and 24 hr on E. faecalis. One-way ANOVA was performed with comparisons to the 0 hr group as the control. (E) Quantification of ilys-3p::GFP at 0, 4, and 24 hr on E. faecalis. One-way ANOVA was performed with comparisons to the 0 hr group as the control.

Figure 3.

Figure 3—figure supplement 1. Loss of key immune genes does not affect avoidance of E. faecalis.

Figure 3—figure supplement 1.

Occupancy index for wild-type, pmk-1(km25), fshr-1(ok778), and bar-1(ga80) on E. faecalis at 4 hr. One-way ANOVA with comparison to the WT group was performed.