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. 2021 May 25;10:e65935. doi: 10.7554/eLife.65935

Figure 8. GON-2 and GTL-2 diminish olfactory aversive learning following E. faecalis exposure.

(A) Occupancy index for WT, gon-2(q388), and gtl-2(n2618) animals with either RNAi-mediated knockdown of aex-5 or an empty vector (EV) control on the E. coli HT115 lawns these animals were raised on. Two-way ANOVA with subsequent comparison between all groups was performed. (B) Occupancy index for WT, gon-2(q388), and gtl-2(n2618) animals with either RNAi-mediated knockdown of aex-5 or an EV control on E. faecalis lawns at 4 hr. Two-way ANOVA with subsequent comparison between all groups was performed. (C) Choice index for wild-type, gtl-2(n2618), gtl-2(tm1463), gon-2(q362), and gon-2(q388) animals between E. faecalis and E. coli using the lid choice assay. Two-way ANOVA with comparison to the naïve and trained WT group was performed. (D) Quantification of learning index for animals from (C). One-way ANOVA with comparison to the WT group was performed. (E) Model for avoidance of P. aeruginosa (top) and E. faecalis (bottom), as in Figure 5E, with the addition of a germline role in avoidance of P. aeruginosa, GON-2 and GTL-2 regulation of avoidance of E. faecalis, and the contribution of odor sensing pathways to avoidance of both bacteria.

Figure 8.

Figure 8—figure supplement 1. Loss of GON-2 or GTL-2 function does not affect anterior intestinal distention on E. faecalis.

Figure 8—figure supplement 1.

(A–C) Representative photomicrographs of the anterior intestines of gtl-2(n2618), gtl-2(tm1463), and gon-2(q388) animals after 4 hr on E. faecalis. Scale bar, 50 μm. (D) Quantification of the anterior intestinal diameters of animals represented in (A–C). One-way ANOVA with subsequent comparison to WT animals was performed.