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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Cell Rep. 2021 May 25;35(8):109184. doi: 10.1016/j.celrep.2021.109184

Figure 4. Increasing autophagy by Becn1F121A does not promote adipogenesis in vivo or in vitro.

Figure 4.

(A) CoIP of endogenous Bcl-2 and Becn1 in the iWAT of WT and Becn1F121A mice. n = 3 mice.

(B) Adipogenesis markers in gWAT and iWAT of RD-fed WT and Becn1F121A mice were analyzed by WB. n = 6 mice. t test. Data represent mean ± SEM.

(C) 3T3-L1 preadipocytes stably expressing hemagglutinin (HA)-tagged WT Becn1 or Becn1F121A were cultured in the adipocyte-differentiation medium for a 10-day time course, and adipogenesis markers were analyzed every 2 days by WB.