Figure 2.

(A) Confirmation of myeloid cell-specific Irf5 deficiency in Bone-marrow derived macrophages (BMDM) isolated from Apoe^–/–Lysm^Cre/+Irf5^fl/flmice (KO) and WT controls (Apoe^−/−Irf5^fl/fl mice) by gel-electrophoresis of Irf5 PCR products. (B) BMDM generated from WT and KO bone marrow cells (M0) were polarized to M1 and M2 subsets in vitro through stimulation with LPS and IFNy or IL-4 and IL-13 for 12 h. Results of expressions for key macrophage-associated genes are presented as mean ± SEM. Gene expressions were analyzed using the ΔΔCq method and normalized to the expression of β-actin. ∗p < 0.05 denotes statistically significant differences between macrophage subtypes, Mann–Whitney, n = 4 per group. IFNγ = interferon, IL = interleukin, LPS = lipopolysaccharide, WT = wild type.