Skip to main content
. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Biol Psychiatry. 2021 Jan 21;89(12):1162–1175. doi: 10.1016/j.biopsych.2021.01.007

Figure 2. Virus expression and local Fos plumes.

Figure 2.

Photomicrograph (×10 magnification) shows channelrhodopsin virus expression (ChR2; green), and neuronal Fos protein expression (magenta) immediately surrounding optic fiber tips for Crh-Cre+ rats in A) nucleus accumbens (NAc) shell, C) lateral division of central nucleus of amygdala (CeA) and E) dorsolateral division of bed nucleus of stria terminalis (BNST). Blue diagrams at right show maps displaying size and intensity of local Fos plumes produced in each structure by laser stimulation of CRF-containing neurons expressing ChR2 (i.e., zones of >150% Fos elevation and >200% Fos elevation over baselines (100%) measured in laser-illuminated eYFP control rats. Average Fos plume diameters are shown for Crh-Cre+ ChR2 rats after laser illumination in B) NAc, D) CeA, and F) BNST CRF-containing neurons. D: dorsal, M: medial, L: lateral, V: ventral to fiber tip; ac, anterior commissure; NAcC, nucleus accumbens core; NAcSh, nucleus accumbens shell; CeC, capsular central amygdala; CeL, lateral central amygdala; CeM, medial central amygdala; dlBNST, dorsolateral bed nucleus of stria terminalis; ic, internal capsule; LV, lateral ventricle.