A) NAc ChR2 rats self-stimulated ~25–35 times on average (n=5 female, n=2 male), whereas NAc eYFP control rats (n=4) touched both spouts equally about 15 times. B) CeA ChR2 rats similarly self-stimulated (n=2 female, n=5 male), whereas eYFP control rats did not (n=4). C) BNST ChR2 rats failed to self-stimulate for laser in BNST CRF-containing neurons (n=3 female, n=5 male). Also see Fig. S4 and Fig. S6D. Means ± SEM, and individual scores shown. *p<0.05,**p<0.01